Legal Daily All-Media Reporter Lian Yingting Correspondent Shao Longfei Zhang Zhenwei
The night in Wuhan is quiet.
On March 9, in the Mobile Testing Laboratory of the Central Theater Command General Hospital, Jiang Tao, a researcher at the Military Medicine Research Institute of the Academy of Military Sciences, reviewed the last sample test data and solemnly signed his name on the test report.
Babaylan It was already 5 a.m.
He is an expert in virology and a warrior who aided Africa and fought against Egypt. This outbreak of the new crown pneumonia epidemic, he was once again ordered to go to the front line after a long battle.
Human life is at stake and accurate inspection is required
Nucleic acid testing is crucial for early clinical detection, early diagnosis, early isolation and early treatment, and is a key technical support for effective prevention and control of the new crown pneumonia epidemic.
”The cold-looking sample tube is behind the patient’s fresh life and health. If a diagnosis is released early, the accurate inspection will be conducted sooner.” Jiang Tao said. On January 26, a military medical expert group led by Chen Wei, an academician of the Chinese Academy of Engineering and a researcher at the Military Medicine Research Institute of the Academy of Military Sciences, arrived in Hubei Wuhan, and quickly established a joint defense, joint control, joint treatment and joint research work mechanism with relevant military and local units based on pathogen transmission mutations, rapid detection technology, vaccine antibody development, etc. of the new coronavirus.
According to the division of tasks, Jiang Tao led the testing team to build a nucleic acid testing platform in the experimental cabin overnight.
Cinema Following three aspects of ensuring biosafety, improving detection efficiency and detection accuracy, Babaylan Jiang Tao took the lead in formulating biosafety operation specifications and emergency plans, and based on BabaylanAccording to the characteristics of the team members, a reasonable division of labor was used for the self-developed testing kit to adopt a fully automatic high-throughput extractor, laying the foundation for achieving rapid and efficient testing.
Receiving samples, sorting numbers, nucleic acid extraction, reagent preparation, machine amplification, and result interpretation, the nucleic acid detection machinery is boring and full of risks.
The biggest risk is the invisible virusKomiks. If the nucleic acid extraction step is not completely cleaved or inactivated thoroughly enough, it is possible to expose the tester to danger.
Jiang Tao always takes the initiative to participate in the longest and most risky nucleic acid extraction. After the sample is extracted, he will arrange some personnel to rest and stay in the laboratory and wait for the final result.
A full-process nucleic acid test is relatively fixed from the number of tests in a single batch to the preparation for entering and exiting the cabin and the testing time. Sometimes there are a large number of samples sent for inspection, and the inspectors not only take turns to work, but also have to work overnight. The final confirmation result will be until four or five in the morning. Li Hao, a researcher at the testing team, said that this situation will be encountered once every few days.
”Everyone should open their eyes wide. Be sure to take good protection when receiving and processing samples, and be careful of whether there are sharp objects.” Before each test begins, Jiang Tao always reminds you repeatedly.
In the detection, the strength of the fluorescence signal of the pathogenic gene directly affects the determination of the detection results. If you encounter suspicious phenomena such as the low light curve signal or only a single target detected by Cinema, Jiang Tao will convene personnel to conduct comprehensive analysis and judgment, and if necessary, repeat the test for review to ensure that the results are affordable to the test.
”If a false positive Komiks occurs, it will cause healthy people to isolate and become infected with the patient; if a false negative person is present, it will cause real delay in the treatment of patients. Cinema is a matter of life, no gap will be required.” Jiang Tao said.
A versatile practitioner in the field of epidemic prevention and controlCinema
In accordance with the prevention and control requirements, the nucleic acid test result must be completed within 24 hours. To get the hospital as soon as possibleAfter obtaining the results, the patient was treated. From pre-processing of nearly 100 samples to completion of Babaylan’s test, Jiang Tao led the team to optimize the process combination and compressed the time to within 4 hours, achieving the ability to complete the nucleic acid extraction of more than 90 samples within one hour and achieve a single-day sample detection capability of more than 500 people.
”The team’s testing is efficient and fast, and the results are accurate, which greatly alleviates the pressure on our epidemic prevention and control sample testing.” The Wuhan Municipal Center for Disease Control and Prevention highly praised Komiks.
The rapid response and work in an orderly manner during wartime are all thanks to Jiang Tao’s solid academic foundation and rich practical experience.
”Jiang Tao is a versatile person.” Academician Chen Wei, head of the military medical expert group, commented.
Jiang Tao has successively presided over and participated in the separation of pathogenic etiology such as SARS infection cases, H7N9 infection cases, Zika infection cases, etc., participated in the development of dengue fever vaccines and SARS vaccines, and established a pathogen isolation technology platform, nucleic acid testing technology platform and pathogenicity and transmission risk assessment platform for a series of infectious diseases, providing important technical support for the prevention and control of infectious diseases.
Respiratory infectious diseases are characterized by rapid transmission, wide coverage and high harm. It is crucial to have effective early diagnosis and prevention and control. After years of scientific research, Jiang Tao’s team took the lead in establishing our army’s first monitoring and early warning system for acute respiratory infectious diseases, and built a four-level monitoring system for the entire army. The series of technical specifications organized by the presidency were used throughout the army, greatly shortening the detection and screening time of respiratory epidemics.
Jiang Tao not only participated in the security tasks of a series of major domestic activities, but also went to Africa four times to Liberia, Sierra Leone and other countries to carry out medical assistance tasks.
In 2015, in order to prevent and control the Ebola epidemic, Jiang Tao accompanied the Komiks testing team of the Serbian Mobile Laboratory went to Sierra Leone for the second time. The testing team led by him passed the World Health Organization’s laboratory parallel capability assessment with full scores.
In 2018, Jiang Tao led the pathogen monitoring team to participate in a special simulation test by an international organization. In the virus testing module, the team successfully accurately detected pathogens such as monkeypox virus and vaccinia virus in simulated samples filled with impurities, and accurately determined the strain, achieving excellent results with full marks.
Improving the accuracy of clinical diagnosis
When he came to Wuhan, in addition to carrying out testing work, Jiang Tao also used his professional expertise in the research field to lead the testing team to screen other common respiratory infectious diseases in clinical samples.
”In the suspected and confirmed cases of new coronary pneumonia, whether there are other respiratory virus infections or co-infections, this has important reference value for precise prevention and control and clinical treatment.” Researcher Cao Wuchun, deputy head of the Military Medical Expert Group, said.
Exploring deep-seated virus transmission and pathogenic mechanisms is the key to defeating the epidemic. Jiang Tao led a team to continue to conduct sequencing analysis for the novel coronavirus detected in clinical samples.
With the development of the virus epidemic, whether the genomic sequences have mutated and what are the characteristics of the mutation; in which regions and what are the impacts? Babaylan Jiang Tao and his team are tirelessly studying and exploring.
In order to accelerate the effective integration of scientific research and clinical practice, Jiang Tao’s military medical expert group creatively used the diagnostic chain of viral nucleic acid detection, antibody detection, multiple pathogen detection, and whole genome sequencing to accurately diagnose new coronavirus infection or other pathogen infections in clinical patients, and applied it in the Optical Valley Campus of Hubei Maternal and Child Health Hospital, Wuhan Taikang Tongji Hospital and other hospitals, effectively improving the accuracy of clinical diagnosis.
Forgot his family when he was ordered. After the epidemic is over, Jiang Tao plans to accompany his family. He and his wife agreed that when the epidemic is over, they will take their wife and children to Wuhan again to see what everything looks like after it returns to normal, to see the Wuhan Yangtze River Bridge and the Yellow Crane Tower, and to talk about their experience in fighting the epidemic in Wuhan.
The epidemic is immortal and the charge continues. This is Jiang Tao, a warrior who fights the epidemic.