Legal Daily All-Media Reporter Lian Yingting Communications Babaylan Member Shao Longfei Zhang Zhenwei
The night in Wuhan is quiet.
On March 9, in the mobile testing laboratory of the Central Theater Command General Hospital, Jiang Tao, a researcher at the Military Medicine Research Institute of the Academy of Military Sciences, reviewed the last sample test data and solemnly signed his name on the test report.
At this time, it was already 5 a.m.
He is an expert in virology and is also a warrior who aided Africa and fought against Egypt. This outbreak of the new crown pneumonia epidemic, he was once again ordered to go to the front line after a long battle.
Human life is at stake
Nucleic acid testingBabaylan is crucial for early clinical diagnosis, early diagnosis, early isolation and early treatment, and is a key technical support for effective prevention and control of the new coronary pneumonia epidemic.
”Behind the cold-looking sample tube is the life and health of the patient. If a diagnosis is released early, the accurate inspection will be conducted sooner.” Jiang Tao said. On January 26, after a military medical expert group led by Chen Komiks Wei, an academician of the Chinese Academy of Engineering and a researcher at the Military Medicine Research Institute of the Academy of Military Sciences, arrived in Wuhan, Hubei, he quickly established a joint defense, joint control, joint treatment and joint research work mechanism with relevant military and local units around the pathogen transmission mutation, rapid detection technology, vaccine antibody development, etc. of the new coronavirus.
According to the division of tasks, Jiang Tao led the testing team to build a nucleic acid testing platform in the experimental cabin overnight.
Following the three aspects of ensuring biosafety, improving detection efficiency and detection accuracy, Jiang Tao took the lead in formulating the full operation of biosafety Cinema specifications and emergency plans, reasonably conducting division of labor according to the characteristics of the team members, and using a self-developed detection kit to adopt a fully automatic high-throughput extractor, laying the foundation for achieving rapid and efficient detection.
Receiving samples, sorting numbers, nucleic acid extraction, reagent preparation, machine amplification, and result interpretation, the nucleic acid detection machinery is boring and full of risks.
The biggest riskIt is an invisible virus. If the nucleic acid extraction step is not completely cleaved or inactivated thoroughly enough, it is possible to expose the tester to danger.
Jiang Tao always takes the initiative to participate in the longest and most risky nucleic acid extraction. After the sample is extracted, he will arrange some personnel to rest and stay in the laboratory and wait for the final result.
A full-process nucleic acid test is relatively fixed from the number of tests in a single batch to the preparation for entering and exiting the cabin and the testing time. Sometimes there are a large number of samples sent for inspection, and the inspectors not only take turns to work, but also have to work overnight. The final confirmation result will be until four or five in the morning. Li Hao, a researcher at the testing team, said that this situation will be encountered once every few days.
”Everyone should open their eyes wide, and take good protection when receiving and processing samples, and pay attention to whether there are sharp objects.” Before each test begins, Jiang Tao always reminds you repeatedly.
In the detection, the strength of the fluorescence signal of the pathogenic gene directly affects the determination of the detection results. If there are suspicious phenomena such as the detected fluorescence curve signal is low or there is only a single target, Jiang Tao will convene personnel to conduct comprehensive analysis and judgment, and if necessary, repeat the test for review to ensure that the results can stand the test.
”A false positive will cause healthy people to isolate and become infected with the patient; a false negative will cause the treatment of real patients to be delayed. Life is a matter of life, and there is no gap.” Jiang Tao said.
All-rounded people in the field of epidemic prevention and control
According to prevention and control requirements, the nucleic acid test results must be completed within 24 hours after receiving the pharyngeal swab sample. In order to let the hospital know the results as soon as possible to treat patients, Jiang Tao led the team to optimize the process combination to reduce the time to less than 4 hours, and achieved the single-day sample extraction of this nucleic acid and the maximum number of 500 samples in one hour.
”The team’s testing is efficient and fast, and the results are accurate, which greatly alleviates the pressure on our epidemic prevention and control sample testing.” The Wuhan Center for Disease Control and Prevention highly praised this.
The rapid response and orderly work during wartime are all thanks to Jiang Tao’s solid academic foundation and rich practical experience.
”Jiang Tao is a versatile person.” Academician Chen Wei, head of the military medical expert group, commented.
Jiang TaoCinema has successively presided over the pathogen separation of SARS infection cases, H7N9 infection cases, Zika infection cases, etc., participated in the development of dengue fever vaccines and SARS vaccines, and established a pathogen isolation technology platform, nucleic acid testing technology platform and pathogenicity and transmission risk assessment platform for a series of infectious diseases, providing important technical support for the prevention and control of infectious diseases.
Respiratory infectious diseases are characterized by rapid transmission, wide coverage, and high harm. Effective early diagnosis and prevention are crucial. After years of scientific research, Jiang Tao’s team took the lead in establishing our army’s first monitoring and early warning system for acute respiratory infectious diseases, and built a four-level monitoring system for the entire army. The series of technical specifications compiled by the presidency were used throughout the army, greatly shortening the detection and screening time of respiratory epidemics.
Jiang Tao not only participated in the security tasks of a series of major domestic activities, but also went to Africa four times to Liberia, Sierra Leone and other countries to carry out medical assistance tasks. In 2015, in order to prevent and control the outbreak of EboCinema, Jiang Tao went to Sierra Leone for the second time with the mobile laboratory testing team to assist in Seychelles. The testing team led by him passed the parallel laboratory capability assessment organized by the World Health Organization with full scores.
In 2018, Jiang Tao led the pathogen monitoring team to participate in a special simulation test by an international organization. In the virus testing module, the team successfully accurately detected pathogens such as monkeypox virus and cowpox disease in simulated samples filled with impurities, and accurately determined the strain, achieving excellent results with full marks.
Improving the accuracy of clinical diagnosis
Come to Wuhan, exceptIn addition to conducting testing, Jiang Tao also used his professional expertise in the research field to lead the testing team to screen other common respiratory infectious diseases in clinical samples.
”In the suspected and confirmed cases of COVID-19, whether there are other respiratory virus infections or co-infections, this is of great reference value for precise prevention and control and clinical treatment.” Researcher Cao Wuchun, deputy head of the Military Medical Expert Group, said.
Exploring deep-seated virus transmission and pathogenic mechanisms is the key to defeating the epidemic. Regarding the novel coronavirus detected in clinical samples, Jiang Tao led his team to continue to conduct sequencing analysis.
As the development of the virus epidemic, whether the genomic sequences have mutated and what are the characteristics of the mutations; in which regions and what are the impacts, Jiang Tao and his team are tirelessly studying and exploring.
In order to quickly promote the effective integration of scientific research and clinical practice, Jiang Tao’s military medical expert group creatively used the diagnostic chain of viral nucleic acid testing, antibody testing, multiple pathogen testing, and whole genome sequencing to accurately diagnose clinical patients with new coronavirus infection or other pathogen infections, and applied it in hospitals such as the Optical Valley Campus of Hubei Maternal and Child Health Hospital, Taikang Tongji Hospital in Wuhan, effectively improving the accuracy of clinical diagnosis.
Forgot his family when he was ordered. After the epidemic is over, Jiang Tao plans to accompany his family. He and his wife agreed that when the epidemic is over, they will take their wife and children to Wuhan again to see what everything looks like after it returns to normal, to see the Wuhan Yangtze River Bridge and the Yellow Crane Tower, and to talk about their experience in fighting the epidemic in Wuhan.
The epidemicKomiksThe charge continues to last. This is Jiang Tao, a warrior who fought the epidemic.